Students are advised to practice the NCERT MCQ Questions for Class 12 Biology Chapter 11 Biotechnology: Principles and Processes with Answers Pdf free download is available here. MCQ Questions for Class 12 Biology with Answers are prepared as per the Latest Exam Pattern. Students can solve these Biotechnology: Principles and Processes Class 12 MCQs Questions with Answers and assess their preparation level.
Biotechnology: Principles and Processes Class 12 MCQs Questions with Answers
Solving the Biotechnology: Principles and Processes Multiple Choice Questions of Class 12 Biology Chapter 11 MCQ can be of extreme help as you will be aware of all the concepts. These MCQ Questions on Biotechnology: Principles and Processes Class 12 with answers pave for a quick revision of the Chapter thereby helping you to enhance subject knowledge. Have a glance at the MCQ of Chapter 11 Biology Class 12 and cross-check your answers during preparation.
Select the correct answer
Question 1.
Rising of dough is due to
(a) Multiplication of yeast
(b) Production of CO2
(c) Emulsification
(d) Hydrolysis of wheat flour starch into sugars.
Answer
Answer: (b) Production of CO2
Question 2.
An enzyme catalysing the removal of nucleotides from the ends of DNA is:
(a) endonuclease
(b) exonuclease
(c) DNAligase
(d) Hind-ll.
Answer
Answer: (b) exonuclease
Question 3.
The transfer of genetic material from one bacterium to another through the mediation of a vector-like virus is termed as:
(a) Transduction
(b) Conjugation
(c) Transformation
(d) Translation.
Answer
Answer: (a) Transduction
Question 4.
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
(a) DNA can be seen in visible light
(b) DNA can be seen without staining in visible light
(c) Ethidium bromide stained DNA can be seen in visible light
(d) Ethidium bromide stained DNA can be seen under exposure to UV light.
Answer
Answer: (d) Ethidium bromide stained DNA can be seen under exposure to UV light.
Question 5.
‘Restriction’ in Restriction enzyme refers to:
(a) Cleaving of phosphodiester bond in DNA by the enzyme
(b) Cutting of DNA at specific position only
(c) Prevention of the multiplication of bacteriophage in bacteria
(d) All of the above.
Answer
Answer: (d) All of the above.
Question 6.
A recombinant DNA molecule can be produced in the absence of the following:
(a) Restriction endonuclease
(b) DNA ligase
(c) DNA fragments
(d) E. coli.
Answer
Answer: (d) E. coli.
Question 7.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
(a) Charge only
(b) Size only
(c) Charge to size ratio
(d) All of the above.
Answer
Answer: (b) Size only
Question 8.
The most important feature in a plasmid to be used as a vector is:
(a) Origin of replication (ori)
(b) Presence of a selectable marker
(c) Presence of sites for restriction endonuclease
(d) Its size.
Answer
Answer: (a) Origin of replication (ori)
Question 9.
While isolating DNA from bacteria, which of the following enzymes is not used?
(a) Lyozyme
(b) Ribonuclease
(c) Deoxyribonuclease
(d) Protease.
Answer
Answer: (c) Deoxyribonuclease
Question 10.
Which of the following has popularised the PCR (polymerase chain reactions)?
(a) Easy availability of DNA template
(b) Availability of synthetic primers
(c) Availability of cheap deoxyribonucleotides
(d) Availability of ‘Thermostable’ DNA polymerase.
Answer
Answer: (d) Availability of ‘Thermostable’ DNA polymerase.
Question 11.
An antibiotic resistance gene in a vector usually helps in the selection of:
(a) Competent cells
(b) Transformed cells
(c) Recombinant cells
(d) None of the above.
Answer
Answer: (b) Transformed cells
Question 12.
Significance of ‘heat shock’ method in bacterial transformation is to facilitate:
(a) Binding of DNA to the cell wall
(b) Uptake of DNA through membrane transport proteins
(c) Uptake of DNA through transisent pores in the bacterial cell wall and plasma membrane
(d) Expression of antibiotic resistance gene.
Answer
Answer: (c) Uptake of DNA through transisent pores in the bacterial cell wall and plasma membrane
Question 13.
Name the enzymes ‘P’ and ‘Q’ that are involved in the processes given below.
(a) Enzyme P-Exonuclease and Enzyme Q-Permease
(b) Enzyme P-Exonuclease and Enzyme Q-Ligase
(c) Enzyme P-Endonuclease and Enzyme Q-Permease
(d) Enzyme P-Restriction endonuclease and Enzyme Q-Ligase
Answer
Answer: (d) Enzyme P-Restriction endonuclease and Enzyme Q-Ligase
Question 14.
A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?
(a) Sal I
(b) Pvu I
(c) EcoRI
(d) Hind III
Answer
Answer: (a) Sal I
Question 15.
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
(a) Denaturation of template DNA
(b) Annealing of primers to template DNA
(c) Extension of primer end on the template DNA
(d) All of the above.
Answer
Answer: (c) Extension of primer end on the template DNA
Question 16.
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
(a) Human gene may have intron which bacteria cannot process
(b) Amino acid codon for humans and bacteria are different
(c) Human protein is formed but degraded by bacteria
(d) All of the above.
Answer
Answer: (a) Human gene may have intron which bacteria cannot process
Question 17.
Which of the following should be chosen for best yield if one were to produce a recombinnt protein in large amount?
(a) Laboratory flask of largest capacity
(b) A stirred-tank bioreactor without in-lets and outlets
(c) A continuous culture system
(d) Any of the above.
Answer
Answer: (c) A continuous culture system
Question 18.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
(a) Herbert Boyer
(b) Hargobind Khorana
(c) KaryMullis
(d) Arthur Kornberg.
Answer
Answer: (c) KaryMullis
Question 19.
Which of the following statements does not hold true for restriction enzyme?
(a) It recognises a palindromic nucleotide sequence.
(b) It is an endonuclease.
(c) It is isolated from viruses.
(d) It produes the same kind of sticky ends in different DNA molecules.
Answer
Answer: (c) It is isolated from viruses.
Assertion and Reason Type Questions
These questions consist of two statements each, printed as Assertion and Reason.
While answering these questions you are required to choose any one of the following four responses:
(a) If both Assertion and Reason are true and Reason is a correct explanation of the As-sertion.
(b) If both Assertion and Reason are true but Reason is not a correct explanation of the Assertion.
(c) If Assertion is true but Reason is false.
(d) If both Assertion and Reason are false.
Question 20.
Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzyme.
Reason: In pBR 322 alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.
Answer
Answer: (c) If Assertion is true but Reason is false.
Question 21.
Assertion: Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure.
Reason: It requires simultaneous planting on two plates having different antibiotics.
Answer
Answer: (a) If both Assertion and Reason are true and Reason is a correct explanation of the As-sertion.
Question 22.
Assertion: Genetic engineering is basically the alteration of the genetic make up of cells by deliberate and artificial means.
Reason: It involves transfer or replacement of genes to create recombinant DNA.
Answer
Answer: (a) If both Assertion and Reason are true and Reason is a correct explanation of the As-sertion.
Question 23.
Assertion: Three main types of restriction endonucleases, i.e. Type I, Type II and Type III are known with slightly different mode of action.
Reason: In palindromes with rotational symmetry second half of complementary strand in DNA double helix is the mirror image of base sequence in the first half of another strand.
Answer
Answer: (c) If Assertion is true but Reason is false.
Question 24.
Assertion: Genetic engineering is also called recombinant DNA technology.
Reason: It brings about improvement of genetic make up of an organism.
Answer
Answer: (a) If both Assertion and Reason are true and Reason is a correct explanation of the As-sertion.
Question 25.
Assertion: DNA segments can be excised by ‘molecular scissors or chemical scalpals’ what biotechnologists call restriction enzymes.
Reason: Restriction enzymes synthesised by microbes as a defence mechanism are specific endonucleases which can cleave double-stranded DNA.
Answer
Answer: (a) If both Assertion and Reason are true and Reason is a correct explanation of the As-sertion.
Question 26.
Assertion: Antibodies have proved effective in lowering mortality rate.
Reason: Antibodies are used as preservation of food like fresh meat, fish and poultry feed.
Answer
Answer: (b) If both Assertion and Reason are true but Reason is not a correct explanation of the Assertion.
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